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rat mab anti egr1  (R&D Systems)


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    Structured Review

    R&D Systems rat mab anti egr1
    Rat Mab Anti Egr1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+mab+anti+egr1/Human+EGR1+Antibody/pmc12546648-114-15-20
    Average 93 stars, based on 3 article reviews
    rat mab anti egr1 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Formalin-fixed Paraffin-Embedded:

    Article Title: Transcriptomic analysis identifies a potential target for bevacizumab resistant glioblastoma.
    Article Snippet: This was followed by reaction with the three secondary antibodies (1 h at 22 °C): donkey anti-rat Alexa FluorTM Plus 488 (5 μg/ml, Thermo A48269), donkey anti-mouse Alexa FluorTM Plus 647 (5 μg/ml, Thermo A32787), and donkey anti-rabbit Alexa FluorTM 568 (5 μg/ml, Thermo A10042), and DAPI nuclear stain. .. Triple-labeling for EGR1, Ki67 and human nucleoli on FFPE sections utilized the following primary antibodies: rat mAb anti-EGR1 (20 μg/ml, R&D Systems MAB28181), rabbit anti-Ki67 (4.5 μg/ml, Invitrogen, PAS-19462), and mouse mAb anti-human nucleoli (5.5 μg/ml, Abcam ab190710), and the primary antibodies were incubated overnight at 4 °C. .. This was followed by reaction with the three secondary antibodies (1 h at 22 °C): donkey anti-rat Alexa FluorTM Plus 488 (5 μg/ml, Thermo A48269), donkey anti-rabbit Alexa FluorTM Plus 647 (5 μg/ml, Thermo 32795), and donkey anti-mouse Alexa FluorTM 568 (5 μg/ml, Thermo A10037), and DAPI nuclear stain.

    Article Title: Transcriptomic analysis identifies a potential target for bevacizumab resistant glioblastoma.
    Article Snippet: Sections were stained for SOX10 with rabbit anti-SOX10 (1.5 μg/ml, Sigma-Aldrich, 383 A-74) after permeabilization with 0.25% Triton-X-100 for 15 min. Rabbit IgG was substituted for the first antibody as the negative control. .. Triple-labeling for EGR1, Ki67 and human nuclei on frozen and on FFPE sections Triple-labeling for EGR1, Ki67 and human nuclei on frozen sections utilized the following primary antibodies: rat mAb anti-EGR1 (20 μg/ml, R&D Systems MAB28181), rabbit anti-human nuclei (2 μg/ml, clone 235-1R, Fig. 2. ..

    Article Title: Transcriptomic analysis identifies a potential target for bevacizumab resistant glioblastoma
    Article Snippet: This was followed by reaction with the three secondary antibodies (1 h at 22 °C): donkey anti-rat Alexa Fluor TM Plus 488 (5 μg/ml, Thermo A48269), donkey anti-mouse Alexa Fluor TM Plus 647 (5 μg/ml, Thermo A32787), and donkey anti-rabbit Alexa Fluor TM 568 (5 μg/ml, Thermo A10042), and DAPI nuclear stain. .. Triple-labeling for EGR1, Ki67 and human nucleoli on FFPE sections utilized the following primary antibodies: rat mAb anti-EGR1 (20 μg/ml, R&D Systems MAB28181), rabbit anti-Ki67 (4.5 μg/ml, Invitrogen, PAS-19462), and mouse mAb anti-human nucleoli (5.5 μg/ml, Abcam ab190710), and the primary antibodies were incubated overnight at 4 °C. .. This was followed by reaction with the three secondary antibodies (1 h at 22 °C): donkey anti-rat Alexa Fluor TM Plus 488 (5 μg/ml, Thermo A48269), donkey anti-rabbit Alexa Fluor TM Plus 647 (5 μg/ml, Thermo 32795), and donkey anti-mouse Alexa Fluor TM 568 (5 μg/ml, Thermo A10037), and DAPI nuclear stain.

    Incubation:

    Article Title: Transcriptomic analysis identifies a potential target for bevacizumab resistant glioblastoma.
    Article Snippet: This was followed by reaction with the three secondary antibodies (1 h at 22 °C): donkey anti-rat Alexa FluorTM Plus 488 (5 μg/ml, Thermo A48269), donkey anti-mouse Alexa FluorTM Plus 647 (5 μg/ml, Thermo A32787), and donkey anti-rabbit Alexa FluorTM 568 (5 μg/ml, Thermo A10042), and DAPI nuclear stain. .. Triple-labeling for EGR1, Ki67 and human nucleoli on FFPE sections utilized the following primary antibodies: rat mAb anti-EGR1 (20 μg/ml, R&D Systems MAB28181), rabbit anti-Ki67 (4.5 μg/ml, Invitrogen, PAS-19462), and mouse mAb anti-human nucleoli (5.5 μg/ml, Abcam ab190710), and the primary antibodies were incubated overnight at 4 °C. .. This was followed by reaction with the three secondary antibodies (1 h at 22 °C): donkey anti-rat Alexa FluorTM Plus 488 (5 μg/ml, Thermo A48269), donkey anti-rabbit Alexa FluorTM Plus 647 (5 μg/ml, Thermo 32795), and donkey anti-mouse Alexa FluorTM 568 (5 μg/ml, Thermo A10037), and DAPI nuclear stain.

    Article Title: Transcriptomic analysis identifies a potential target for bevacizumab resistant glioblastoma.
    Article Snippet: .. Double labeling for EGR1 and α7-nAChR for IF studies This utilized the following primary antibodies: rat mAb anti-EGR1 (20 μg/ml, R&D Systems MAB28181) and rabbit anti-α7-nAChR (1.5 μg/ml, Abcam ab216485), with incubation overnight at 4 °C. .. This was followed by reaction with the two secondary antibodies (1 h at 22 °C): donkey anti-rat Alexa FluorTM Plus 488 (5 μg/ ml, Thermo A48269), and sheep anti-rabbit HRP followed by Opal Tyramide 570 Amplifier (1:1000, Akoya Biosciences FP1488001KT), and DAPI nuclear stain.

    Article Title: Transcriptomic analysis identifies a potential target for bevacizumab resistant glioblastoma
    Article Snippet: .. This utilized the following primary antibodies: rat mAb anti-EGR1 (20 μg/ml, R&D Systems MAB28181) and rabbit anti-α7-nAChR (1.5 μg/ml, Abcam ab216485), with incubation overnight at 4 °C. .. This was followed by reaction with the two secondary antibodies (1 h at 22 °C): donkey anti-rat Alexa Fluor TM Plus 488 (5 μg/ml, Thermo A48269), and sheep anti-rabbit HRP followed by Opal Tyramide 570 Amplifier (1:1000, Akoya Biosciences FP1488001KT), and DAPI nuclear stain.

    Article Title: Transcriptomic analysis identifies a potential target for bevacizumab resistant glioblastoma
    Article Snippet: This was followed by reaction with the three secondary antibodies (1 h at 22 °C): donkey anti-rat Alexa Fluor TM Plus 488 (5 μg/ml, Thermo A48269), donkey anti-mouse Alexa Fluor TM Plus 647 (5 μg/ml, Thermo A32787), and donkey anti-rabbit Alexa Fluor TM 568 (5 μg/ml, Thermo A10042), and DAPI nuclear stain. .. Triple-labeling for EGR1, Ki67 and human nucleoli on FFPE sections utilized the following primary antibodies: rat mAb anti-EGR1 (20 μg/ml, R&D Systems MAB28181), rabbit anti-Ki67 (4.5 μg/ml, Invitrogen, PAS-19462), and mouse mAb anti-human nucleoli (5.5 μg/ml, Abcam ab190710), and the primary antibodies were incubated overnight at 4 °C. .. This was followed by reaction with the three secondary antibodies (1 h at 22 °C): donkey anti-rat Alexa Fluor TM Plus 488 (5 μg/ml, Thermo A48269), donkey anti-rabbit Alexa Fluor TM Plus 647 (5 μg/ml, Thermo 32795), and donkey anti-mouse Alexa Fluor TM 568 (5 μg/ml, Thermo A10037), and DAPI nuclear stain.

    Article Title: Transcriptomic analysis identifies a potential target for bevacizumab resistant glioblastoma
    Article Snippet: Sections were stained for SOX10 with rabbit anti-SOX10 (1.5 μg/ml, Sigma-Aldrich, 383 A-74) after permeabilization with 0.25% Triton-X-100 for 15 min. Rabbit IgG was substituted for the first antibody as the negative control. .. Triple-labeling for EGR1, Ki67 and human nuclei on frozen sections utilized the following primary antibodies: rat mAb anti-EGR1 (20 μg/ml, R&D Systems MAB28181), rabbit anti-human nuclei (2 μg/ml, clone 235-1R, Neobiotechnologies RBM5-346-P1), and mouse mAb anti-Ki67 (1:2500, Cell Signaling #9449) and the first antibodies were incubated overnight at 4 °C. .. This was followed by reaction with the three secondary antibodies (1 h at 22 °C): donkey anti-rat Alexa Fluor TM Plus 488 (5 μg/ml, Thermo A48269), donkey anti-mouse Alexa Fluor TM Plus 647 (5 μg/ml, Thermo A32787), and donkey anti-rabbit Alexa Fluor TM 568 (5 μg/ml, Thermo A10042), and DAPI nuclear stain.

    Staining:

    Article Title: Transcriptomic analysis identifies a potential target for bevacizumab resistant glioblastoma.
    Article Snippet: .. Sections were stained for EGR1 with rat mAb anti-EGR1 (20 μg/ml, R&D Systems #MAB28181) after permeabilization with 0.25% Triton X-100 for 5-min. Rat IgG was substituted for the first antibody as the negative control. .. Sections were stained for α7-nAChR with rabbit monoclonal anti-α7-nAChR (1.5 μg/ml, Abcam ab216485) after permeabilization with 0.25% Triton X-100 for 5 min, and endogenous peroxidase blocking with 0.3% hydrogen peroxide for 20 min.

    Article Title: Transcriptomic analysis identifies a potential target for bevacizumab resistant glioblastoma
    Article Snippet: .. Sections were stained for EGR1 with rat mAb anti-EGR1 (20 μg/ml, R&D Systems #MAB28181) after permeabilization with 0.25% Triton X-100 for 5-min. Rat IgG was substituted for the first antibody as the negative control. .. Sections were stained for α7-nAChR with rabbit monoclonal anti-α7-nAChR (1.5 μg/ml, Abcam ab216485) after permeabilization with 0.25% Triton X-100 for 5 min, and endogenous peroxidase blocking with 0.3% hydrogen peroxide for 20 min.

    Negative Control:

    Article Title: Transcriptomic analysis identifies a potential target for bevacizumab resistant glioblastoma.
    Article Snippet: .. Sections were stained for EGR1 with rat mAb anti-EGR1 (20 μg/ml, R&D Systems #MAB28181) after permeabilization with 0.25% Triton X-100 for 5-min. Rat IgG was substituted for the first antibody as the negative control. .. Sections were stained for α7-nAChR with rabbit monoclonal anti-α7-nAChR (1.5 μg/ml, Abcam ab216485) after permeabilization with 0.25% Triton X-100 for 5 min, and endogenous peroxidase blocking with 0.3% hydrogen peroxide for 20 min.

    Article Title: Transcriptomic analysis identifies a potential target for bevacizumab resistant glioblastoma
    Article Snippet: .. Sections were stained for EGR1 with rat mAb anti-EGR1 (20 μg/ml, R&D Systems #MAB28181) after permeabilization with 0.25% Triton X-100 for 5-min. Rat IgG was substituted for the first antibody as the negative control. .. Sections were stained for α7-nAChR with rabbit monoclonal anti-α7-nAChR (1.5 μg/ml, Abcam ab216485) after permeabilization with 0.25% Triton X-100 for 5 min, and endogenous peroxidase blocking with 0.3% hydrogen peroxide for 20 min.

    Labeling:

    Article Title: Transcriptomic analysis identifies a potential target for bevacizumab resistant glioblastoma.
    Article Snippet: .. Double labeling for EGR1 and α7-nAChR for IF studies This utilized the following primary antibodies: rat mAb anti-EGR1 (20 μg/ml, R&D Systems MAB28181) and rabbit anti-α7-nAChR (1.5 μg/ml, Abcam ab216485), with incubation overnight at 4 °C. .. This was followed by reaction with the two secondary antibodies (1 h at 22 °C): donkey anti-rat Alexa FluorTM Plus 488 (5 μg/ ml, Thermo A48269), and sheep anti-rabbit HRP followed by Opal Tyramide 570 Amplifier (1:1000, Akoya Biosciences FP1488001KT), and DAPI nuclear stain.



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